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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
17/05/2022 |
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Actualizado : |
02/12/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
PASSOS, J. R. S.; GUERREIRO, D. D.; OTÁVIO, K. S.; SANTOS-NETO, P. C. DOS; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ-OLIVERA, R.; CRISPO, M.; BEZERRA, M. J. B.; SILVA, R. F.; LIMA, L. F.; FIGUEIREDO, J. R.; BUSTAMANTE-FILHO, I. C.; MENCHACA, A.; MOURA, A. A. |
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Afiliación : |
JOSÉ RENATO S. PASSOS, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; DENISE D. GUERREIRO, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; KAMILA S. OTÁVIO, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; P. C. DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; FEDERICO CUADRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; RICHARD NUÑEZ-OLIVERA, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; MARTINA CRISPO, Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Montevideo, Uruguay; MARIA JÚLIA B. BEZERRA, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; RENATO F. SILVA, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; LARITZA F. LIMA, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; JOSÉ RICARDO FIGUEIREDO, Laboratório de Manipulação de Oócitos e Folículos Ovarianos Pré-antrais - LAMOFOPA - Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Brazil; IVAN C. BUSTAMANTE-FILHO, aboratório de Biotecnologia da Reprodução Animal, Programa de Pós-graduação em Biotecnologia, Universidade do Vale do Taquari, Lajeado, Brazil; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; ARLINDO A. MOURA, Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil. |
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Título : |
Global proteomic analysis of preimplantational ovine embryos produced in vitro. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Reproduction in Domestic Animals, 2022, Volume 57, Issue 7; pages 784-797. doi: https://doi.org/10.1111/rda.14122 |
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ISSN : |
0936-6768 |
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DOI : |
10.1111/rda.14122 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 15 February 2022; Accepted 1 April 2022. -- Funding text - The experiments presently described were conducted at the facilities of the (Fundacion IRAUy, Montevideo, Uruguay) and at the (UBAL) of the , Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Finnacial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); the Brazilian Research Council?CNPq (grants # 313160/2017‐1 and 438773/2018‐7); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil. Instituto de Reproducción Animal Uruguay Unidad de Biotecnología en Animales de Laboratorio Institut Pasteur de Montevideo. -- Corresponding author: A. Moura, A.; Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; mail:menchaca.alejo@gmail.com |
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Contenido : |
ABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine
embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters
according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational initiation, cell body and nucleotide biosynthetic process. The most representative KEEG pathways were ribosome, oxida- tive phosphorylation, glutathione metabolism, gap junction, mineral absorption, DNA replication and cGMP-PKG signalling pathway. Analyses of functional clusters clearly showed differences associated
with the proteome of preimplantation (D6) sheep em- bryos generated after in vitro fertilization in comparison with in vivo counterparts (Sanchez et al., 2021; https://doi.org/10.1111/rda.13897), confirming that the quality of in vitro derived blastocysts are unlike those produced in vivo. The present study portrays the first comprehensive overview of the proteome of preimplantational ovine embryos grown in vitro.
© 2022 Wiley-VCH GmbH. MenosABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine
embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters
according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational i... Presentar Todo |
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Palabras claves : |
Embryo development; In vitro fertilization; Mass spectrometry; Oocyte; Ovine; PLATAFORMA SALUD ANIMAL; Proteins. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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Marc : |
LEADER 04571naa a2200409 a 4500
001 1063149
005 2022-12-02
008 2022 bl uuuu u00u1 u #d
022 $a0936-6768
024 7 $a10.1111/rda.14122$2DOI
100 1 $aPASSOS, J. R. S.
245 $aGlobal proteomic analysis of preimplantational ovine embryos produced in vitro.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 15 February 2022; Accepted 1 April 2022. -- Funding text - The experiments presently described were conducted at the facilities of the (Fundacion IRAUy, Montevideo, Uruguay) and at the (UBAL) of the , Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Finnacial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); the Brazilian Research Council?CNPq (grants # 313160/2017‐1 and 438773/2018‐7); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil. Instituto de Reproducción Animal Uruguay Unidad de Biotecnología en Animales de Laboratorio Institut Pasteur de Montevideo. -- Corresponding author: A. Moura, A.; Laboratório de Fisiologia e Ciências Ômicas, Departamento de Zootecnia, Universidade Federal do Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Menchaca, A.; Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay; mail:menchaca.alejo@gmail.com
520 $aABSTRACT. -The present study was conducted to characterize the major proteome of preimplantation (D6) ovine embryos produced in vitro. COCs were aspirated from antral follicles (2–6 mm), matured and fertilized in vitro and cultured until day six. Proteins were ex- tracted separately from three pools of 45 embryos and separately run in SDS-PAGE. Proteins from each pool were individually subjected to in-gel digestion followed by LC-MS/MS. Three ‘raw files’ and protein lists were produced by Pattern Lab software, but only proteins present in all three lists were used for the bioinformatics analyses. There were 2,262 proteins identified in the 6-day-old ovine embryos, including al- bumin, zona pellucida glycoprotein 2, 3 and 4, peptidyl arginine deiminase 6, actin cytoplasmic 1, gamma-actin 1, pyruvate kinase, heat shock protein 90 and protein disulfide isomerase, among others. Major biological processes linked to the sheep embryo proteome were translation, protein transport and protein stabilization, and molecular functions, defined as ATP binding, oxygen carrier activity and oxygen bind- ing. There were 42 enriched functional clusters according to the 2,147 genes (UniProt database). Ten selected clusters with potential association with embryo development included translation, structural constituent of ribosomes, ribosomes, nucleosomes, structural constituent of the cytoskeleton, microtubule-based process, translation initiation factor activity, regulation of translational initiation, cell body and nucleotide biosynthetic process. The most representative KEEG pathways were ribosome, oxida- tive phosphorylation, glutathione metabolism, gap junction, mineral absorption, DNA replication and cGMP-PKG signalling pathway. Analyses of functional clusters clearly showed differences associated with the proteome of preimplantation (D6) sheep em- bryos generated after in vitro fertilization in comparison with in vivo counterparts (Sanchez et al., 2021; https://doi.org/10.1111/rda.13897), confirming that the quality of in vitro derived blastocysts are unlike those produced in vivo. The present study portrays the first comprehensive overview of the proteome of preimplantational ovine embryos grown in vitro. © 2022 Wiley-VCH GmbH.
653 $aEmbryo development
653 $aIn vitro fertilization
653 $aMass spectrometry
653 $aOocyte
653 $aOvine
653 $aPLATAFORMA SALUD ANIMAL
653 $aProteins
700 1 $aGUERREIRO, D. D.
700 1 $aOTÁVIO, K. S.
700 1 $aSANTOS-NETO, P. C. DOS
700 1 $aSOUZA-NEVES, M.
700 1 $aCUADRO, F.
700 1 $aNUÑEZ-OLIVERA, R.
700 1 $aCRISPO, M.
700 1 $aBEZERRA, M. J. B.
700 1 $aSILVA, R. F.
700 1 $aLIMA, L. F.
700 1 $aFIGUEIREDO, J. R.
700 1 $aBUSTAMANTE-FILHO, I. C.
700 1 $aMENCHACA, A.
700 1 $aMOURA, A. A.
773 $tReproduction in Domestic Animals, 2022, Volume 57, Issue 7; pages 784-797. doi: https://doi.org/10.1111/rda.14122
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Registro original : |
INIA Las Brujas (LB) |
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Biblioteca
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Clasificación
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Volver
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
29/10/2014 |
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Actualizado : |
08/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CAZZULI, F.; LAGOMARSINO, X.; LUZARDO, S.; MONTOSSI, F. |
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Afiliación : |
FIORELLA CARLA CAZZULI ALBA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; XIMENA MARIA LAGOMARSINO LARRIERA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SANTIAGO FELIPE LUZARDO VILLAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FABIO MARCELO MONTOSSI PORCHILE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
¿Existe respuesta a la suplementación estratégica en novillos de recría pastoreando sudangrás y sorgo forrajero? // Is there a response to strategic supplementation of yearling steers grazing sudangrass and forage sorghum? |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Agro Sur, 2019, v. 47no. 2, p. 7-17. |
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Idioma : |
Español |
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Notas : |
Article history: Received: 09.05.2019 // Accepted: 18.07.2019. |
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Contenido : |
Within the context of sustainable intensification of extensive livestock production systems, the inclusion of reduced areas of high productivity combined with strategic supplementation would allow an animal production increase. Sudangrass and direct grazing sorghum appear as alternatives because of their agroecological adaptation and high forage production. To evaluate the effect of these forage basis combined with the use of protein supplementation on average daily gain (ADG) and live weight (LW), 40 Hereford yearling steers (LW, Year 1 = 287 ± 35 kg; Year 2 = 271 ± 19 kg) were used in a random 2 x 2 factorial design for two years, where forage basis (sudangrass ? Sd ? or BMR sorghum ? Sg) and supplementation (0.8% LW with sunflower expeller: with ? S ? or without ? NOS) were evaluated. The animals were randomly allocated to 4 treatments with two replicates: SdNOS, SdS, SgNOS, and SgS. Average daily gain was significantly affected by forage basis and supplementation (Sd = 0.833 vs. Sg = 0.899 kg animal-1 d-1, P < 0.05; NOS = 0.739 kg animal-1 d-1 vs. S = 0.992 kg animal-1 d-1, P < 0.01), as was final LW (Sd = 342.3 vs. Sd = 347.1 kg, P < 0.05; NOS = 334.7 kg vs. S =354.7 kg; P < 0.01). The use of forage sorghums combined with strategic supplementation improved the rearing of steers during summer // RESUMEN: En el marco de una intensificación sostenible de la ganadería extensiva, la inclusión de áreas reducidas de alta productividad combinada con suplementación estratégica permitiría mejorar la productividad animal. El uso de sudangrás o sorgo forrajero es una alternativa dada su mejor adaptación agroecológica a suelos más marginales y alta producción forrajera. Con el objetivo de evaluar el efecto de estas dos bases forrajeras combinadas con suplementación proteica sobre la ganancia media diaria (GMD) y el peso vivo (PV), por dos años, 40 novillos Hereford de sobreaño (PV, Año 1 = 287 ± 35 kg; Año 2 = 271 ± 19 kg) fueron utilizados en un diseño de parcelas al azar con un arreglo factorial de 2 x 2, en donde se evaluó la base forrajera (sudangrás ? Sd ? o sorgo forrajero BMR ? Sg) y la suplementación (0,8% del PV con expeller de girasol: con ?CS? o sin ?SS). Los novillos fueron asignados al azar a 4 tratamientos con dos repeticiones cada uno: SdSS, SdCS, SgSS y SgCS. La GMD fue afectada significativamente por la base forrajera y suplementación (Sd = 0,833 vs. Sg = 0,899 kg animal-1 d-1, P < 0,05; SS = 0,739 kg animal-1 d-1, CS = 0,992 kg animal-1 d -1, P < 0,01), así como el PV final (Sd = 342,3 vs. Sd = 347,1 kg, P < 0,05; SS = 334,7 kg, CS =354,7 kg, P < 0,01). El uso de sorgos forrajeros con suplementación estratégica mejoró la recría estival de novillos. MenosWithin the context of sustainable intensification of extensive livestock production systems, the inclusion of reduced areas of high productivity combined with strategic supplementation would allow an animal production increase. Sudangrass and direct grazing sorghum appear as alternatives because of their agroecological adaptation and high forage production. To evaluate the effect of these forage basis combined with the use of protein supplementation on average daily gain (ADG) and live weight (LW), 40 Hereford yearling steers (LW, Year 1 = 287 ± 35 kg; Year 2 = 271 ± 19 kg) were used in a random 2 x 2 factorial design for two years, where forage basis (sudangrass ? Sd ? or BMR sorghum ? Sg) and supplementation (0.8% LW with sunflower expeller: with ? S ? or without ? NOS) were evaluated. The animals were randomly allocated to 4 treatments with two replicates: SdNOS, SdS, SgNOS, and SgS. Average daily gain was significantly affected by forage basis and supplementation (Sd = 0.833 vs. Sg = 0.899 kg animal-1 d-1, P < 0.05; NOS = 0.739 kg animal-1 d-1 vs. S = 0.992 kg animal-1 d-1, P < 0.01), as was final LW (Sd = 342.3 vs. Sd = 347.1 kg, P < 0.05; NOS = 334.7 kg vs. S =354.7 kg; P < 0.01). The use of forage sorghums combined with strategic supplementation improved the rearing of steers during summer // RESUMEN: En el marco de una intensificación sostenible de la ganadería extensiva, la inclusión de áreas reducidas de alta productividad combinada con suplementación estratégica p... Presentar Todo |
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Palabras claves : |
BMR SORGHUM FORAGE CROP; EXPELLER DE GIRASOL; NOVILLOS; SORGO FORRAJERO BMR; STEERS; SUDANGRÁS; SUDANGRASS FORAGE CROP; SUNFLOWER EXPELLER; SUPLEMENTACIÓN; SUPPLEMENTATION. |
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Thesagro : |
PRODUCCIÓN ANIMAL. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13455/1/Cazzuli-2019.pdf
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Marc : |
LEADER 03758nam a2200289 a 4500
001 1051365
005 2019-10-08
008 2019 bl uuuu u00u1 u #d
100 1 $aCAZZULI, F.
245 $a¿Existe respuesta a la suplementación estratégica en novillos de recría pastoreando sudangrás y sorgo forrajero? // Is there a response to strategic supplementation of yearling steers grazing sudangrass and forage sorghum?$h[electronic resource]
260 $aAgro Sur, 2019, v. 47no. 2, p. 7-17.$c2019
500 $aArticle history: Received: 09.05.2019 // Accepted: 18.07.2019.
520 $aWithin the context of sustainable intensification of extensive livestock production systems, the inclusion of reduced areas of high productivity combined with strategic supplementation would allow an animal production increase. Sudangrass and direct grazing sorghum appear as alternatives because of their agroecological adaptation and high forage production. To evaluate the effect of these forage basis combined with the use of protein supplementation on average daily gain (ADG) and live weight (LW), 40 Hereford yearling steers (LW, Year 1 = 287 ± 35 kg; Year 2 = 271 ± 19 kg) were used in a random 2 x 2 factorial design for two years, where forage basis (sudangrass ? Sd ? or BMR sorghum ? Sg) and supplementation (0.8% LW with sunflower expeller: with ? S ? or without ? NOS) were evaluated. The animals were randomly allocated to 4 treatments with two replicates: SdNOS, SdS, SgNOS, and SgS. Average daily gain was significantly affected by forage basis and supplementation (Sd = 0.833 vs. Sg = 0.899 kg animal-1 d-1, P < 0.05; NOS = 0.739 kg animal-1 d-1 vs. S = 0.992 kg animal-1 d-1, P < 0.01), as was final LW (Sd = 342.3 vs. Sd = 347.1 kg, P < 0.05; NOS = 334.7 kg vs. S =354.7 kg; P < 0.01). The use of forage sorghums combined with strategic supplementation improved the rearing of steers during summer // RESUMEN: En el marco de una intensificación sostenible de la ganadería extensiva, la inclusión de áreas reducidas de alta productividad combinada con suplementación estratégica permitiría mejorar la productividad animal. El uso de sudangrás o sorgo forrajero es una alternativa dada su mejor adaptación agroecológica a suelos más marginales y alta producción forrajera. Con el objetivo de evaluar el efecto de estas dos bases forrajeras combinadas con suplementación proteica sobre la ganancia media diaria (GMD) y el peso vivo (PV), por dos años, 40 novillos Hereford de sobreaño (PV, Año 1 = 287 ± 35 kg; Año 2 = 271 ± 19 kg) fueron utilizados en un diseño de parcelas al azar con un arreglo factorial de 2 x 2, en donde se evaluó la base forrajera (sudangrás ? Sd ? o sorgo forrajero BMR ? Sg) y la suplementación (0,8% del PV con expeller de girasol: con ?CS? o sin ?SS). Los novillos fueron asignados al azar a 4 tratamientos con dos repeticiones cada uno: SdSS, SdCS, SgSS y SgCS. La GMD fue afectada significativamente por la base forrajera y suplementación (Sd = 0,833 vs. Sg = 0,899 kg animal-1 d-1, P < 0,05; SS = 0,739 kg animal-1 d-1, CS = 0,992 kg animal-1 d -1, P < 0,01), así como el PV final (Sd = 342,3 vs. Sd = 347,1 kg, P < 0,05; SS = 334,7 kg, CS =354,7 kg, P < 0,01). El uso de sorgos forrajeros con suplementación estratégica mejoró la recría estival de novillos.
650 $aPRODUCCIÓN ANIMAL
653 $aBMR SORGHUM FORAGE CROP
653 $aEXPELLER DE GIRASOL
653 $aNOVILLOS
653 $aSORGO FORRAJERO BMR
653 $aSTEERS
653 $aSUDANGRÁS
653 $aSUDANGRASS FORAGE CROP
653 $aSUNFLOWER EXPELLER
653 $aSUPLEMENTACIÓN
653 $aSUPPLEMENTATION
700 1 $aLAGOMARSINO, X.
700 1 $aLUZARDO, S.
700 1 $aMONTOSSI, F.
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